An unusual feruloyl esterase belonging to family VIII esterases and displaying a broad substrate range
نویسندگان
چکیده
منابع مشابه
Expanding the feruloyl esterase gene family of Aspergillus niger by characterization of a feruloyl esterase, FaeC.
A feruloyl esterase (FAE) from Aspergillus niger N402, FaeC was heterologously produced in Pichia pastoris X-33 in a yield of 10mg/L. FaeC was most active at pH 7.0 and 50°C, and showed broad substrate specificity and catalyzed the hydrolysis of methyl 3,4-dimethoxycinnamate, ethyl ferulate, methyl ferulate, methyl p-coumarate, ethyl coumarate, methyl sinapate, and methyl caffeate. The enzyme r...
متن کاملFeruloyl esterase
Feruloyl esterase forms a part of the enzyme complex that acts collectively and synergistically to completely hydrolyze xylan to its monomers. The enzyme has found potential uses in a wide variety of applications of interest to the agrifood and pharmaceutical industries. This review describes the enzymology of feruloyl esterases involved in xylan degradation. The occurrence of feruloyl esterase...
متن کاملA spectrophotometric assay for feruloyl esterases.
We have developed a spectrophotometric assay for the quantitative determination of feruloyl esterase activity based on release of 4-nitrophenol from a novel substrate, 4-nitrophenyl ferulate in an emulsion of Triton X-100 in aqueous buffer solution. The release of 4-nitrophenol was linear with reaction time at an early stage of the reaction with various esterase preparations. The method propose...
متن کاملSpecificity of ferulic acid (feruloyl) esterases.
Specificity of ferulic acid (feruloyl) esterases G. Williamson’, C. B. Faulds and P. A. Kroon Biochemistry Department, Institute of Food Research, Norwich Research Park, Colney, Norwich NR4 7UA, U.K. 205 moyl esters (manifest as a K,,, of between lop6 Feruloyl esterases are a subclass of the carboxylic ester hydrolases (EC 3.1.1.1) which hydrolyse ester linkages between hydroxycinnamates and su...
متن کاملXylanolytic enzymes : X Feruloyl esterases : Pectinases : Phytases :
Table 1 Thermophilic moulds are a potential reservoir of thermozymes which find potential applications in various biotechnological processes (Singh , 2016; Singh, 2016; Maheshwari 2000; Johri 1999). Enzymes from thermophilic fungi often tolerate higher temperatures than enzymes from their mesophilic counterparts and some show stability even at 70-80°C (Singh 2016; Singh, 2016; Johri 1999). It h...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Molecular Catalysis B: Enzymatic
سال: 2015
ISSN: 1381-1177
DOI: 10.1016/j.molcatb.2015.04.010